Phospho-MSK1 (Thr581) Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3284R-Biotin-100uL |
| Product Name | Phospho-MSK1 (Thr581) Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Thr581 [LK(p-T)PC] |
| Modification Site | Thr581 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 9252 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | MSK1 Phospho Thr581; 90 kDa ribosomal protein S6 kinase 5; EC 2.7.11.1; KS6A5_HUMAN; MGC1911; Mitogen and stress activated protein kinase 1; MSPK1; Nuclear Mitogen And Stress Activated Protein Kinase 1; Nuclear mitogen- and stress-activated protein kinase 1; Ribosomal protein S6 kinase 90kD polypeptide 5; Ribosomal protein S6 kinase 90kDa; Ribosomal protein S6 kinase 90kDa polypeptide 5; Ribosomal Protein S6 Kinase Alpha 5; Ribosomal protein S6 kinase alpha-5; RLPK; RPS6KA5; RSK Like Protein Kinase; RSK-like protein kinase; RSKL; S6K alpha 5; S6K-alpha-5. |
| Background | MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG14 in response to mitogen and stress. Similar to RSK, MSK1 contains two kinase domains (N term and a C term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Phospho-MSK1 (Thr581) Polyclonal Antibody, Biotin Conjugated (bs-3284R-Biotin) validated for, and at what dilution?
bs-3284R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-MSK1 (Thr581) Polyclonal Antibody, Biotin Conjugated (bs-3284R-Biotin) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Chicken, Rabbit.
How should bs-3284R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-MSK1 (Thr581) Polyclonal Antibody, Biotin Conjugated (bs-3284R-Biotin)?
KLH conjugated synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Thr581 [LK(p-T)PC]