Phospho-MYPT1 (Thr696) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3287R-100uL |
| Product Name | Phospho-MYPT1 (Thr696) Polyclonal Antibody |
| Applications |
WB
FCM
|
| Specificity | This phosphorylation site is homologous to that of Thr694 in Mouse and Thr697 in Rat. The region containing this phosphorylation site is known to be homologous to that of the related protein, PP12C, and will react with this protein when phosphorylated at Thr560 in Human and Mouse, and Thr561 in Rat. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of Ser696 |
| Modification Site | Thr696 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4659 |
| Swiss Prot | O14974 |
| Subcellular location | Cytoplasm |
| Synonyms | MBS; M130; MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit; PPP1R12A |
| Background | Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-MYPT1 (Thr696) Polyclonal Antibody (bs-3287R) validated for, and at what dilution?
bs-3287R is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-MYPT1 (Thr696) Polyclonal Antibody (bs-3287R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Horse, Chicken, Rabbit.
How should bs-3287R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-MYPT1 (Thr696) Polyclonal Antibody (bs-3287R)?
KLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of Ser696