Phospho-Mre11 (Ser676) Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3293R-BF594 |
| Product Name | Phospho-Mre11 (Ser676) Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from rat Mre11 around the phosphorylation site of Ser676 |
| Modification Site | Ser676 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4361 |
| Subcellular location | Nucleus |
| Synonyms | Mre 11; MRE 11a; MRE 11b; MRE11 homolog 1; MRE11 meiotic recombination 11 homolog A; MRE11a; MRE11b; AT like disease; Ataxia telangiectasia disorder like; Ataxia-telangiectasia disorder-like; ATLD; DNA recombination and repair protein; Double strand break repair protein MRE11A; Double-strand break repair protein MRE11A; endo/exonuclease Mre11. HNGS1; meiotic recombination S. cerevisiae 11 homolog A; meiotic recombination 11 homolog A S. cerevisiae; meiotic recombination 11 homolog A; MmMRE11A.MRE11_RAT |
| Background | Mre11 is a component of the MRN complex, which plays a critical role in double-strand break repair (DSB), DNA recombination, maintenance of telomere integrity, and meiosis. Mre11 has both single-strand endonuclease and double strand-specific 3'-5' exonuclease activities. Rad50, another component of the MRN complex, is postulated to bind to DNA ends and hold them in close proximity, thus facilitating searches for regions of sequence homology, and also possibly stimulating the activity of DNA ligases and/or restricting Mre11 activity. Mutations in Mre11 may cause breast cancer. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Mre11 (Ser676) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-3293R-BF594) validated for, and at what dilution?
bs-3293R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Mre11 (Ser676) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-3293R-BF594) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog.
How should bs-3293R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Mre11 (Ser676) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-3293R-BF594)?
KLH conjugated synthetic phosphopeptide derived from rat Mre11 around the phosphorylation site of Ser676
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-3293R-BF594)?
AbBy Fluor-594 on bs-3293R-BF594: excitation / emission 590nm/617nm.