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GFP Polyclonal Antibody

50 ng rGFP-His protein (bs-33009P) per lane probed with GFP polyclonal antibody respectively, unconjugated (bs-33009R) at 1:10000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
50 ng rGFP-His protein (bs-33009P) per lane probed with GFP polyclonal antibody respectively, unconjugated (bs-33009R) at 1:10000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
20 ng rGFP-His protein (bs-33009P) per lane probed with GFP polyclonal antibody respectively, unconjugated (bs-33009R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
20 ng rGFP-His protein (bs-33009P) per lane probed with GFP polyclonal antibody respectively, unconjugated (bs-33009R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
GFP tag was immunoprecipitated from GFP expression vector whole cell lysate with bs-33009R at 1\/150 dilution. Western blot was performed from the immunoprecipitate using protein A\/G beads. HRP conjugated Goat Anti-Rabbit IgG (H&L) was used as secondary antibody.\r\nLane 1: GFP expression vector whole cell lysate (Input).\r\nLane 2: bs-33009R IP in GFP expression vector whole cell lysate.\r\nLane 3: native rabbit IgG IP in GFP expression vector whole cell lysate (negative control).\r\n
GFP tag was immunoprecipitated from GFP expression vector whole cell lysate with bs-33009R at 1\/150 dilution. Western blot was performed from the immunoprecipitate using protein A\/G beads. HRP conjugated Goat Anti-Rabbit IgG (H&L) was used as secondary antibody.\r\nLane 1: GFP expression vector whole cell lysate (Input).\r\nLane 2: bs-33009R IP in GFP expression vector whole cell lysate.\r\nLane 3: native rabbit IgG IP in GFP expression vector whole cell lysate (negative control).\r\n
Bioss unconjugated antibody vial

Applications

  • WB

Reactivity

  • Belt Jellyfish
  • Species independent
Overview
Catalog # bs-33009r
Product Name GFP Polyclonal Antibody
Applications
WB
Reactivity Belt Jellyfish, Species independent
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant Green Fluorescence Protein
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms Green fluorescence protein; GFP Tag; GFPTag
Background Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin
Application Dilution
WB =1:500-2000

Frequently asked questions

Which applications is GFP Polyclonal Antibody (bs-33009R) validated for, and at what dilution?

bs-33009R is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GFP Polyclonal Antibody (bs-33009R) react with?

Tested reactivity: Belt Jellyfish, Species independent.

How should bs-33009R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GFP Polyclonal Antibody (bs-33009R)?

Recombinant Green Fluorescence Protein

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