Phospho-NFKB1 (Ser927) Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3300R-BF647-100uL |
| Product Name | Phospho-NFKB1 (Ser927) Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Hamster |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from rat NFKB1 around the phosphorylation site of Ser477:ND(p-S)VC. |
| Modification Site | Ser927 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4790 |
| Swiss Prot | Q63369 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DKFZp686C01211; DNA binding factor KBF1; DNA binding factor KBF1 EBP1; DNA binding factor KBF1 EBP1; DNA-binding factor KBF1; EBP 1; EBP-1; EBP1; KBF1; MGC54151; NF kappa B; NF kappabeta; NF kB1; NFKB 1; NFKB p105; NFKB p50; NFKB1; Nuclear factor kappa B DNA binding subunit; Nuclear factor NF kappa B p105 subunit; Nuclear factor NF kappa B p50 subunit; Nuclear factor NF-kappa-B p50 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; p84/NF-kappa-B1 p98. |
| Background | This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-NFKB1 (Ser927) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3300R-BF647) validated for, and at what dilution?
bs-3300R-BF647 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-NFKB1 (Ser927) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3300R-BF647) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Hamster.
How should bs-3300R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-NFKB1 (Ser927) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3300R-BF647)?
KLH conjugated synthetic phosphopeptide derived from rat NFKB1 around the phosphorylation site of Ser477:ND(p-S)VC.
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-3300R-BF647)?
AbBy Fluor-647 on bs-3300R-BF647: excitation / emission 650nm/665nm.