Strep-Tag II Antibody, PE-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-33016R-PE-Cy5-100uL |
| Product Name | Strep-Tag II Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide of Strep-Tag |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted |
| Synonyms | Strep; Strep Tag; Strep.Tag; Strep-Tactin; |
| Background | The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Strep-Tag II Antibody, PE-Cy5 Conjugated (bs-33016R-PE-Cy5) validated for, and at what dilution?
bs-33016R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Strep-Tag II Antibody, PE-Cy5 Conjugated (bs-33016R-PE-Cy5) react with?
Tested reactivity: Species independent.
How should bs-33016R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Strep-Tag II Antibody, PE-Cy5 Conjugated (bs-33016R-PE-Cy5)?
KLH conjugated synthetic peptide of Strep-Tag
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-33016R-PE-Cy5)?
PE-Cy5 on bs-33016R-PE-Cy5: excitation / emission 488nm/670nm.