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Phospho-NMDAR1 (Ser890) Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Chicken
Overview
Catalog # bs-3301R-APC-Cy7-100uL
Product Name Phospho-NMDAR1 (Ser890) Polyclonal Antibody, APC-Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This phosphorylation site is homologous to that of Ser890 in Mouse and Rat.
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Chicken
Specifications
Conjugation APC-Cy7
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human NMDAR1 around the phosphorylation site of Ser890
Modification Site Ser890
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2902
Swiss Prot Q05586
Subcellular location Cell membrane
Synonyms NR1; MRD8; GluN1; NMDA1; NMDAR1; Glutamate receptor ionotropic, NMDA 1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1; GRIN1
Background NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors (By similarity).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Phospho-NMDAR1 (Ser890) Polyclonal Antibody, APC-Cy7 Conjugated (bs-3301R-APC-Cy7) validated for, and at what dilution?

bs-3301R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-NMDAR1 (Ser890) Polyclonal Antibody, APC-Cy7 Conjugated (bs-3301R-APC-Cy7) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Chicken.

How should bs-3301R-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-NMDAR1 (Ser890) Polyclonal Antibody, APC-Cy7 Conjugated (bs-3301R-APC-Cy7)?

KLH conjugated synthetic phosphopeptide derived from human NMDAR1 around the phosphorylation site of Ser890

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-3301R-APC-Cy7)?

APC-Cy7 on bs-3301R-APC-Cy7: excitation / emission 650nm/780nm.