Phospho-NMDAR2B (Tyr1070) Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3306R-APC-Cy7-100uL |
| Product Name | Phospho-NMDAR2B (Tyr1070) Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylated site is homologous to that of Tyr1070 in Mouse and Rat. |
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human NMDAR2B around the phosphorylation site of Tyr1070 |
| Modification Site | Tyr1070 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2904 |
| Swiss Prot | Q13224 |
| Subcellular location | Cell membrane |
| Synonyms | MRD6; NR2B; hNR3; GluN2B; NMDAR2B; Glutamate receptor ionotropic, NMDA 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NR3; GRIN2B |
| Background | NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-NMDAR2B (Tyr1070) Polyclonal Antibody, APC-Cy7 Conjugated (bs-3306R-APC-Cy7) validated for, and at what dilution?
bs-3306R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3306R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-NMDAR2B (Tyr1070) Polyclonal Antibody, APC-Cy7 Conjugated (bs-3306R-APC-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human NMDAR2B around the phosphorylation site of Tyr1070
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-3306R-APC-Cy7)?
APC-Cy7 on bs-3306R-APC-Cy7: excitation / emission 650nm/780nm.