Phospho-NUMA1 (Ser395) Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3312R-Cy5-100uL |
| Product Name | Phospho-NUMA1 (Ser395) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human NuMA around the phosphorylation site of Ser395 |
| Modification Site | Ser395 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4926 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | NUMA 1; NuMA protein; NUMA1; SP H antigen; Structural nuclear protein; Centrophilin stabilizes mitotic spindle in mitotic cells; Nuclear mitotic apparatus protein 1; Nuclear mitotic apparatus protein. |
| Background | NuMA (Nuclear Mitotic Apparatus Protein) is an intranuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-NUMA1 (Ser395) Polyclonal Antibody, Cy5 Conjugated (bs-3312R-Cy5) validated for, and at what dilution?
bs-3312R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3312R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-NUMA1 (Ser395) Polyclonal Antibody, Cy5 Conjugated (bs-3312R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human NuMA around the phosphorylation site of Ser395
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3312R-Cy5)?
Cy5 on bs-3312R-Cy5: excitation / emission 625,650nm/670nm.