S1-Tag Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-33206R-Cy5-100uL |
| Product Name | S1-Tag Antibody, Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide S1-Tag |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | S1 Tag; S1Tag; NANNPDWDF; |
| Background | Epitope tagging is a recombinant DNA method by which a protein encoded by a cloned gene is made immunoreactive to a known antibody. Major areas ofapplication include monitoring protein expression, localizing proteins at thecellular and subcellular levels, and protein purification, as well as the analysis of protein topology, dynamics and interactions. Due to their small size, theyare unlikely to affect the tagged protein's biochemical properties.S1 tag is an epitope tag composed of a nine-residue peptide, NANNPDWDF, derived from the hepatitis B virus preS1 region. Epitope tags consisting ofshort sequences recognized by well-characterizated antibodies have beenwidely used in the study of protein expression in various systems. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is S1-Tag Antibody, Cy5 Conjugated (bs-33206R-Cy5) validated for, and at what dilution?
bs-33206R-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does S1-Tag Antibody, Cy5 Conjugated (bs-33206R-Cy5) react with?
Tested reactivity: Species independent.
How should bs-33206R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise S1-Tag Antibody, Cy5 Conjugated (bs-33206R-Cy5)?
KLH conjugated synthetic peptide S1-Tag
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-33206R-Cy5)?
Cy5 on bs-33206R-Cy5: excitation / emission 625,650nm/670nm.