Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857) Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3320R-PE-Cy5-100uL |
| Product Name | Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857) Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PDGF Receptor alpha around the phosphorylation site of Tyr849 [SN(p-Y)VS] |
| Modification Site | Tyr857 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5156 |
| Subcellular location | Cell membrane |
| Synonyms | PDGF Receptor alphaTyr849/PDGF Receptor beta Tyr857; Plateletdirived growth factor receptor-alpha; Alpha platelet derived growth factor receptor; CD 140a; CD140a; CD140a antigen; MGC74795; PDGF alpha chain; PDGF R alpha; PDGFR 2; PDGFR A; PDGFR alpha; PDGFR2; PDGFRA; Platelet derived growth factor receptor 2; Platelet derived growth factor receptor alpha; Platelet derived growth factor receptor alpha polypeptide; PGFRA_HUMAN. |
| Background | The PDGF Receptor Type A (Alpha platelet-derived growth factor receptor precursor, CD140a antigen), a 170kD protein, binds all three isoforms of PDGF with high affinity whereas the PDGF Receptor Type B, a 190kD protein, appears to bind only the PDGF BB homodimer with high affinity. Both receptors are transmembranous, ligand activated protein tyrosine kinases, which phosphorylate a number of important signal transduction proteins, which are bound with differential affinities via SH2 domains. The response of any given cell to PDGF will depend on the types of receptors displayed on the surface and isoforms of PDGF present in the extracellular environment. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3320R-PE-Cy5) validated for, and at what dilution?
bs-3320R-PE-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3320R-PE-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken.
How should bs-3320R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3320R-PE-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human PDGF Receptor alpha around the phosphorylation site of Tyr849 [SN(p-Y)VS]
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-3320R-PE-Cy5)?
PE-Cy5 on bs-3320R-PE-Cy5: excitation / emission 488nm/670nm.