Phospho-PBK (Thr9) Polyclonal Antibody

$Lane 1: Mouse testis lysates probed with PBK\/TOPK(Thr9) Polyclonal Antibody, Unconjugated (bs-3326R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.$

Lane 1: Mouse testis lysates probed with PBK\/TOPK(Thr9) Polyclonal Antibody, Unconjugated (bs-3326R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse

Predicted Reactivity

Human
Rat

Overview
Catalog #	bs-3326R
Product Name	Phospho-PBK (Thr9) Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse
Predicted Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human PBK/TOPK around the phosphorylation site of Thr9
Modification Site	Thr9
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55872
Swiss Prot	Q96KB5
Subcellular location	Nucleus
Synonyms	SPK; CT84; TOPK; HEL164; Nori-3; Lymphokine-activated killer T-cell-originated protein kinase; Cancer/testis antigen 84; MAPKK-like protein kinase; PDZ-binding kinase; Spermatogenesis-related protein kinase; T-LAK cell-originated protein kinase; PBK
Background	Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200