Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3331R-RBITC-100uL |
| Product Name | Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylated site is homologous to that of Ser467 in Mouse and Ser496 in Rat. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PFK2 around the phosphorylation site of Ser467 |
| Modification Site | Ser467 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5209 |
| Swiss Prot | Q16875 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | PFK2; IPFK2; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; 6PF-2-K/Fru-2,6-P2ase 3; PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; Renal carcinoma antigen NY-REN-56; iPFK-2; PFKFB3 |
| Background | 6 Phosphofructo 2 Kinase plays a role in the synthesis and degradation of fructose 2,6-bisphosphate. Fructose-2,6-bisphosphate acts as an allosteric regulator of 6-phosphofructo-1-kinase (PFK1), which catalyses a key step in the glycoytic pathway. Glycolytic flux is key to tumour growth, and small molecule inhibition of 6 Phosphofructo 2 Kinase suppresses both glycolytic flux and tumour growth. Several lines of evidence suggest that 6 Phosphofructo 2 Kinase is also involved in obesity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody, RBITC Conjugated (bs-3331R-RBITC) validated for, and at what dilution?
bs-3331R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody, RBITC Conjugated (bs-3331R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse, Chicken, Rabbit.
How should bs-3331R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PFKFB3/PFK2 (Ser467) Polyclonal Antibody, RBITC Conjugated (bs-3331R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human PFK2 around the phosphorylation site of Ser467
What are the excitation and emission wavelengths of the RBITC conjugate (bs-3331R-RBITC)?
RBITC on bs-3331R-RBITC: excitation / emission 540nm/625nm.