phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3332R-RBITC-100uL |
| Product Name | phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PI3K around the phosphorylation site of p85 alphaTyr467 |
| Modification Site | Tyr467 + Tyr199 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5295 |
| Swiss Prot | P27986 |
| Subcellular location | Cytoplasm |
| Synonyms | p85; AGM7; GRB1; IMD36; p85-ALPHA; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; PIK3R1 |
| Background | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p11 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Polyclonal Antibody, RBITC Conjugated (bs-3332R-RBITC) validated for, and at what dilution?
bs-3332R-RBITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Polyclonal Antibody, RBITC Conjugated (bs-3332R-RBITC) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Horse, Rabbit.
How should bs-3332R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Polyclonal Antibody, RBITC Conjugated (bs-3332R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human PI3K around the phosphorylation site of p85 alphaTyr467
What are the excitation and emission wavelengths of the RBITC conjugate (bs-3332R-RBITC)?
RBITC on bs-3332R-RBITC: excitation / emission 540nm/625nm.