Phospho-PKM2 (Tyr105) Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3334R-Biotin-100uL |
| Product Name | Phospho-PKM2 (Tyr105) Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Rat, Rabbit |
| Predicted Reactivity | Mouse, Dog, Cow, Pig, Horse, GuineaPig |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PKM2 around the phosphorylation site of Tyr105 |
| Modification Site | Tyr105 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 5315 |
| Swiss Prot | P14618 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Pyruvate kinase PKM; Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58; PKM; OIP3; PK2; PK3; PKM2 |
| Background | Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Phospho-PKM2 (Tyr105) Polyclonal Antibody, Biotin Conjugated (bs-3334R-Biotin) validated for, and at what dilution?
bs-3334R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PKM2 (Tyr105) Polyclonal Antibody, Biotin Conjugated (bs-3334R-Biotin) react with?
Tested reactivity: Human, Rat, Rabbit. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Horse, GuineaPig.
How should bs-3334R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PKM2 (Tyr105) Polyclonal Antibody, Biotin Conjugated (bs-3334R-Biotin)?
KLH conjugated synthetic phosphopeptide derived from human PKM2 around the phosphorylation site of Tyr105