Phospho-PLCG 2 (Tyr1217) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3338R-Cy5-100uL |
| Product Name | Phospho-PLCG 2 (Tyr1217) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rabbit |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, GuineaPig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PLC gamma 2 around the phosphorylation site of Tyr1217 |
| Modification Site | Tyr1217 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5336 |
| Subcellular location | Cytoplasm |
| Synonyms | PLCG2phospho Y1217; PLC _2Tyr1217; PLC gamma 2/PLCG2Tyr1217; PLC 2; PLC gamma 2; PLC IV; PLCG2; 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2; EC 3.1.4.11; Phosphoinositide phospholipase C; Phospholipase C gamma 2; Phospholipase C, gamma 2 phosphatidylinositol specic; PLCG2_HUMAN. |
| Background | Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water soluble phosphorylated derivatives of the lipid head groups. A number of these enzymes have specificity for phosphoinositides. Of the phosphoinositide specific phospholipase C enzymes, C beta is regulated by heterotrimeric G protein coupled receptors, while the closely related C gamma 1 and C gamma 2 enzymes are controlled by receptor tyrosine kinases. The C gamma 1 and C gamma 2 enzymes are composed of phospholipase domains that flank regions of homology to noncatalytic domains of the SRC oncogene product, SH2 and SH3. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-PLCG 2 (Tyr1217) Polyclonal Antibody, Cy5 Conjugated (bs-3338R-Cy5) validated for, and at what dilution?
bs-3338R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PLCG 2 (Tyr1217) Polyclonal Antibody, Cy5 Conjugated (bs-3338R-Cy5) react with?
Tested reactivity: Human, Rabbit. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, GuineaPig.
How should bs-3338R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PLCG 2 (Tyr1217) Polyclonal Antibody, Cy5 Conjugated (bs-3338R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human PLC gamma 2 around the phosphorylation site of Tyr1217
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3338R-Cy5)?
Cy5 on bs-3338R-Cy5: excitation / emission 625,650nm/670nm.