Phospho-PLCB3 (Ser1105) Polyclonal Antibody, PE-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3341R-PE-Cy7-100uL |
| Product Name | Phospho-PLCB3 (Ser1105) Polyclonal Antibody, PE-Cy7 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PLC beta3 around the phosphorylation site of Ser1105 |
| Modification Site | Ser1105 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5331 |
| Subcellular location | Cell membrane |
| Synonyms | Phospholipase C beta 3; Phospholipase C beta 3 phosphatidylinositol specic; PLC beta 3; PLCB3 antibody 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3; Phosphoinositide phospholipase C; PLCB3_HUMAN. |
| Background | Phospholipase C is a key enzyme in phosphatidylinositol (PIP2) metabolism and lipid signaling pathways. The Phospholipase C family consists of 13 isozymes split between six subfamilies. Phospholipase C beta is activated by G protein subunits. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-PLCB3 (Ser1105) Polyclonal Antibody, PE-Cy7 Conjugated (bs-3341R-PE-Cy7) validated for, and at what dilution?
bs-3341R-PE-Cy7 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PLCB3 (Ser1105) Polyclonal Antibody, PE-Cy7 Conjugated (bs-3341R-PE-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse.
How should bs-3341R-PE-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PLCB3 (Ser1105) Polyclonal Antibody, PE-Cy7 Conjugated (bs-3341R-PE-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human PLC beta3 around the phosphorylation site of Ser1105
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bs-3341R-PE-Cy7)?
PE-Cy7 on bs-3341R-PE-Cy7: excitation / emission 488nm/780nm.