Phospho-PLCG1 (Tyr783) Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3343R-BF680-100uL |
| Product Name | Phospho-PLCG1 (Tyr783) Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous across the listed species. |
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PLC gamma 1 around the phosphorylation site of Tyr783 |
| Modification Site | Tyr783 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5335 |
| Swiss Prot | P19174 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; Phospholipase C-gamma-1; PLC-gamma-1; PLCG1 |
| Background | Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-PLCG1 (Tyr783) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-3343R-BF680) validated for, and at what dilution?
bs-3343R-BF680 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PLCG1 (Tyr783) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-3343R-BF680) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Pig, Horse, Chicken.
How should bs-3343R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PLCG1 (Tyr783) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-3343R-BF680)?
KLH conjugated synthetic phosphopeptide derived from human PLC gamma 1 around the phosphorylation site of Tyr783
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-3343R-BF680)?
AbBy Fluor-680 on bs-3343R-BF680: excitation / emission 679nm/702nm.