Phospho-Paxillin (Tyr118) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3352R-Cy5 |
| Product Name | Phospho-Paxillin (Tyr118) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
|
| Specificity | This phosphorylation site is homologous to Tyr118 in Mouse and Rat. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr118 |
| Modification Site | Tyr118 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5829 |
| Swiss Prot | P49023 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Paxillin; PXN |
| Background | Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Phospho-Paxillin (Tyr118) Polyclonal Antibody, Cy5 Conjugated (bs-3352R-Cy5) validated for, and at what dilution?
bs-3352R-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Paxillin (Tyr118) Polyclonal Antibody, Cy5 Conjugated (bs-3352R-Cy5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Horse, Chicken, Rabbit.
How should bs-3352R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Paxillin (Tyr118) Polyclonal Antibody, Cy5 Conjugated (bs-3352R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr118
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3352R-Cy5)?
Cy5 on bs-3352R-Cy5: excitation / emission 625,650nm/670nm.