Phospho-Paxillin (Tyr118) Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3352R-HRP |
| Product Name | Phospho-Paxillin (Tyr118) Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
|
| Specificity | This phosphorylation site is homologous to Tyr118 in Mouse and Rat. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr118 |
| Modification Site | Tyr118 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5829 |
| Swiss Prot | P49023 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Paxillin; PXN |
| Background | Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is Phospho-Paxillin (Tyr118) Polyclonal Antibody, HRP Conjugated (bs-3352R-HRP) validated for, and at what dilution?
bs-3352R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Paxillin (Tyr118) Polyclonal Antibody, HRP Conjugated (bs-3352R-HRP) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Horse, Chicken, Rabbit.
How should bs-3352R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Paxillin (Tyr118) Polyclonal Antibody, HRP Conjugated (bs-3352R-HRP)?
KLH conjugated synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr118