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Phospho-Paxillin (Tyr118) Polyclonal Antibody, PE Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-3352R-PE-100uL
Product Name Phospho-Paxillin (Tyr118) Polyclonal Antibody, PE Conjugated
Applications
WB
Specificity This phosphorylation site is homologous to Tyr118 in Mouse and Rat.
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr118
Modification Site Tyr118
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5829
Swiss Prot P49023
Subcellular location Cytoplasm, Cell membrane
Synonyms Paxillin; PXN
Background Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Phospho-Paxillin (Tyr118) Polyclonal Antibody, PE Conjugated (bs-3352R-PE) validated for, and at what dilution?

bs-3352R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-Paxillin (Tyr118) Polyclonal Antibody, PE Conjugated (bs-3352R-PE) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Horse, Chicken, Rabbit.

How should bs-3352R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-Paxillin (Tyr118) Polyclonal Antibody, PE Conjugated (bs-3352R-PE)?

KLH conjugated synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr118

What are the excitation and emission wavelengths of the PE conjugate (bs-3352R-PE)?

PE on bs-3352R-PE: excitation / emission 496,564nm/578nm.