| Overview |
| bs-3373R |
| B-Raf (Ser444) Polyclonal Antibody |
| ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC) |
| Human, Mouse, Rat, Dog, Pig, Horse, Chicken, Rabbit |
| Specifications |
| Unconjugated |
| Rabbit |
| KLH conjugated synthetic phosphopeptide derived from mouse B-Raf around the phosphorylation site of Ser444 |
| Ser444 |
| Polyclonal |
| IgG |
| 1ug/ul |
| Purified by Protein A. |
| 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target |
| 109880 |
| Cytoplasm, Nucleus, Cell membrane |
| 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; BRAF 1; Braf; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral v-raf oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; p94; RAFB 1; RAFB1; v raf murine sarcoma viral oncogene homolog B1; FLJ95109; BRAF_HUMAN. |
| The Raf kinases are important intermediates in signal transduction. Raf protein family members, including A Raf and B Raf, have intrinsic serine/threonine kinase activity. Interaction between Ras proteins and Raf proteins results in Raf-mediated phosphorylation and activation of MEK (also known as MAP kinase kinase). Defects in BRAF are involved in a wide range of cancers. B-Raf is a serine/threonine protein kinase that acts as a signal transducer from membrane-associated receptors to nuclear transcription factors. 1 BRAF is important for the regulation of cell proliferation and determination of cell fate during embryogenesis. BRAF acts downstream of Ras and upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, proliferation, and differentiation in response to growth factors, cytokines, and hormones. |
| Application Dilution |
| ELISA |
1:500-1000 |
| IHC-F |
1:100-500 |
| IF(IHC-P) |
1:50-200 |
| IF(IHC-F) |
1:50-200 |
| IF(ICC) |
1:50-200 |
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