Phospho-Rb (Ser807 + Ser811) Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3380R-APC-Cy7-100uL |
| Product Name | Phospho-Rb (Ser807 + Ser811) Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Chicken, Rabbit |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human P105 RB around the phosphorylation site of Ser807/Ser811 |
| Modification Site | Ser807 + Ser811 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5925 |
| Subcellular location | Nucleus |
| Synonyms | RbSer807; OSRC; P105 RB; P105RB; PP105; PP110; pRb; RB 1; RB1; RB1 protein; Retinoblastoma 1 including osteosarcoma; Retinoblastoma 1; Retinoblastoma associated protein; Retinoblastoma related osteosarcoma; Retinoblastoma susceptibility gene; Including osteosarcoma; RB_HUMAN. |
| Background | Nuclear MarkerRb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Rb (Ser807 + Ser811) Polyclonal Antibody, APC-Cy7 Conjugated (bs-3380R-APC-Cy7) validated for, and at what dilution?
bs-3380R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Rb (Ser807 + Ser811) Polyclonal Antibody, APC-Cy7 Conjugated (bs-3380R-APC-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Chicken, Rabbit.
How should bs-3380R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Rb (Ser807 + Ser811) Polyclonal Antibody, APC-Cy7 Conjugated (bs-3380R-APC-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human P105 RB around the phosphorylation site of Ser807/Ser811
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-3380R-APC-Cy7)?
APC-Cy7 on bs-3380R-APC-Cy7: excitation / emission 650nm/780nm.