Phospho-Raptor (Ser792) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3381R-BF405-100uL |
| Product Name | Phospho-Raptor (Ser792) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Guinea Pig, Zebrafish |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse Raptor around the phosphorylation site of Ser792 |
| Modification Site | Ser792 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | ARaptor phospho S792; Raptor phospho Ser792; p-Raptor Ser792; p150 target of rapamycin TOR scaffold protein containing WD repeats; P150 target of rapamycin TOR-scaffold protein; Regulatory Associated Protein of mTOR; KIAA1303; RPTOR_HUMAN; Regulatory-associated protein of mTOR; p150 target of rapamycin TOR-scaffold protein. |
| Background | mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of Raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Raptor (Ser792) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-3381R-BF405) validated for, and at what dilution?
bs-3381R-BF405 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Raptor (Ser792) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-3381R-BF405) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Guinea Pig, Zebrafish.
How should bs-3381R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Raptor (Ser792) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-3381R-BF405)?
KLH conjugated synthetic phosphopeptide derived from mouse Raptor around the phosphorylation site of Ser792
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-3381R-BF405)?
AbBy Fluor-405 on bs-3381R-BF405: excitation / emission 401nm/421nm.