Phospho-SGK1 (Thr256) Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3397R-BF647-100uL |
| Product Name | Phospho-SGK1 (Thr256) Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human SGK1 around the phosphorylation site of Thr256 |
| Modification Site | Thr256 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6446 |
| Swiss Prot | O00141 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | SGK; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1; SGK1 |
| Background | This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009] |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-SGK1 (Thr256) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3397R-BF647) validated for, and at what dilution?
bs-3397R-BF647 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-SGK1 (Thr256) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3397R-BF647) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-3397R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-SGK1 (Thr256) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3397R-BF647)?
KLH conjugated synthetic phosphopeptide derived from human SGK1 around the phosphorylation site of Thr256
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-3397R-BF647)?
AbBy Fluor-647 on bs-3397R-BF647: excitation / emission 650nm/665nm.