Phospho-SHIP1 (Tyr1020) Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3398R-BF680 |
| Product Name | Phospho-SHIP1 (Tyr1020) Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human SHIP1 around the phosphorylation site of Tyr1020 |
| Modification Site | Tyr1022 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3635 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | P-SHIP1 Tyr1020; Inositol polyphosphate 5 phosphatase of 145kDa; 4; 5-trisphosphate 5-phosphatase 1; hp51CN; hSHIP; Inositol polyphosphate 5 phosphatase 145kDa; Inositol polyphosphate 5 phosphatase; Inositol polyphosphate 5 phosphatase D; Inositol polyphosphate-5-phosphatase of 145 kDa; INPP 5D; INPP5D; INPP5D protein; MGC104855; MGC142140; MGC142142; p150 ship; p150Ship; Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1; Phosphatidylinositol-3; SH2 containing inositol 5 phosphatase; SH2 containing inositol phosphatase isoform b; SH2 domain containing inositol 5' phosphatase 1; SH2 domain containing inositol phosphatase 1; SH2 domain-containing inositol phosphatase 1; SH2 domain-containing inositol-5''-phosphatase 1; SHIP 1; SHIP-1; SHIP1; SHIP1_RAT; Signaling inositol polyphosphate 5 phosphatase SIP 145; Signaling inositol polyphosphate 5 phosphatase SIP145; SIP 145; SIP-145; SIP145. |
| Background | SHIP1 is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and contains an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation in response to multiple cytokine and B and T cell receptor activation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. Overall the protein functions as a negative regulator of myeliod cell proliferation and survival. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-SHIP1 (Tyr1020) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-3398R-BF680) validated for, and at what dilution?
bs-3398R-BF680 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-SHIP1 (Tyr1020) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-3398R-BF680) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig.
How should bs-3398R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-SHIP1 (Tyr1020) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-3398R-BF680)?
KLH conjugated synthetic phosphopeptide derived from human SHIP1 around the phosphorylation site of Tyr1020
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-3398R-BF680)?
AbBy Fluor-680 on bs-3398R-BF680: excitation / emission 679nm/702nm.