phospho-PYK2 (Tyr881) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3401R-Cy3-100uL |
| Product Name | phospho-PYK2 (Tyr881) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Pyk2 around the phosphorylation site of Tyr881 |
| Modification Site | Tyr881 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2185 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | CADTK; CAK beta ; CAKB antibody; CAKbeta; Calcium dependent tyrosine kinase; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; E430023O05Rik; EC 2.7.10.2; FADK 2; FADK2; FAK 2; FAK1; FAK2; Focal Adhesion Kinase 2; MGC124628; PKB; pp125FAK; Proline Rich Tyrosine Kinase 2; Proline-rich tyrosine kinase 2; Protein kinase B; Protein Tyrosine Kinase 2 Beta; PTK 2B; PTK antibody PTK2B; PTK2B protein tyrosine kinase 2 beta; PYK 2; PYK2; RAFTK; Related adhesion focal tyrosine kinase. |
| Background | PYK2 is involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. PKY2 may represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage gated potassium channel protein Kv1.2. PYK2 activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-PYK2 (Tyr881) Polyclonal Antibody, Cy3 Conjugated (bs-3401R-Cy3) validated for, and at what dilution?
bs-3401R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-PYK2 (Tyr881) Polyclonal Antibody, Cy3 Conjugated (bs-3401R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-3401R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PYK2 (Tyr881) Polyclonal Antibody, Cy3 Conjugated (bs-3401R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human Pyk2 around the phosphorylation site of Tyr881
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3401R-Cy3)?
Cy3 on bs-3401R-Cy3: excitation / emission 512,550nm/570,615nm.