Phospho-PTPN11 (Tyr580) Polyclonal Antibody

$Mouse brain lysates probed with Rabbit Anti-PTPN11 (Tyr584) Polyclonal Antibody (bs-3405R) at 1:300 overnight in 4\u02daC. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.$

Mouse brain lysates probed with Rabbit Anti-PTPN11 (Tyr584) Polyclonal Antibody (bs-3405R) at 1:300 overnight in 4\u02daC. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.

$Lane 1: Mouse brain lysates Lane 2: Mouse muscle lysates probed with Anti-PTPN11 (Tyr584) Polyclonal Antibodyd (bs-3405R) at 1:300 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.$

Lane 1: Mouse brain lysates Lane 2: Mouse muscle lysates probed with Anti-PTPN11 (Tyr584) Polyclonal Antibodyd (bs-3405R) at 1:300 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-PTPN11 (Tyr584) Polyclonal Antibody, Unconjugated(bs-3405R) 1:200 followed by conjugation to the secondary antibody and DAB staining

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-PTPN11 (Tyr580)) polyclonal Antibody, Unconjugated (bs-3405R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-PTPN11 (Tyr580)) polyclonal Antibody, Unconjugated (bs-3405R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-PTPN11 (Tyr580) . Antibody(bs-3405R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).$

Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-PTPN11 (Tyr580) . Antibody(bs-3405R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Horse
Chicken

Overview
Catalog #	bs-3405R
Product Name	Phospho-PTPN11 (Tyr580) Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity	This phosphorylation site is homologous across the listed cross reactive species.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human PTPN11 around the phosphorylation site of Tyr584
Modification Site	Tyr584
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5781
Swiss Prot	Q06124
Subcellular location	Cytoplasm
Synonyms	CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3; Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTP-2C; SHP-2; PTPN11; SHPTP2
Background	Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200