Phospho-PTPN11 (Tyr580) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3405R-Cy7 |
| Product Name | Phospho-PTPN11 (Tyr580) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous across the listed cross reactive species. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Horse, Chicken |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PTPN11 around the phosphorylation site of Tyr584 |
| Modification Site | Tyr584 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5781 |
| Swiss Prot | Q06124 |
| Subcellular location | Cytoplasm |
| Synonyms | CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3; Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTP-2C; SHP-2; PTPN11; SHPTP2 |
| Background | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-PTPN11 (Tyr580) Polyclonal Antibody, Cy7 Conjugated (bs-3405R-Cy7) validated for, and at what dilution?
bs-3405R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PTPN11 (Tyr580) Polyclonal Antibody, Cy7 Conjugated (bs-3405R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Horse, Chicken.
How should bs-3405R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PTPN11 (Tyr580) Polyclonal Antibody, Cy7 Conjugated (bs-3405R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human PTPN11 around the phosphorylation site of Tyr584
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-3405R-Cy7)?
Cy7 on bs-3405R-Cy7: excitation / emission 743nm/767nm.