Phospho-SMC1 (Ser957) Polyclonal Antibody

Formalin-fixed and paraffin embedded human endometrial cancer labeled with Anti-Phospho-SMC1(Ser957) Polyclonal Antibody, Unconjugated (bs-3407R) followed by conjugation to the secondary antibody and DAB staining

$HeLa cells probed with Rabbit Anti-SMC1(Ser957) Polyclonal Antibody (bs-3407R) at 1:20 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).\\n$

HeLa cells probed with Rabbit Anti-SMC1(Ser957) Polyclonal Antibody (bs-3407R) at 1:20 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).\\n

Applications

ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Sheep

Overview
Catalog #	bs-3407R
Product Name	Phospho-SMC1 (Ser957) Polyclonal Antibody
Applications	ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity	This phosphorylation site is homologous across the listed species.
Reactivity	Human
Predicted Reactivity	Mouse, Sheep
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human SMC1 around the phosphorylation site of Ser957 [GS(p-S)QG]
Modification Site	Ser957
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8243
Swiss Prot	Q14683
Subcellular location	Nucleus
Synonyms	SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha; Structural maintenance of chromosomes protein 1A; SMC protein 1A; SMC-1-alpha; SMC-1A; SMC1A; KIAA0178
Background	Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Application Dilution
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200