Phospho-NCOA3 (Thr24) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3408R-FITC |
| Product Name | Phospho-NCOA3 (Thr24) Polyclonal Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Pig, Horse, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human SRC-3 around the phosphorylation site of Thr24 |
| Modification Site | Thr24 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8202 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ACTR; AIB 1; AIB-1; AIB1; Amplied in breast cancer 1; Amplied in breast cancer 1 protein; bHLHe42; CAGH 16; CAGH16; CBP interacting protein; CBP-interacting protein; Class E basic helix-loop-helix protein 42; CTG 26; CTG26; KAT13B / AIB1; MGC141848; NCOA 3; NCOA 3; NCoA-3; Ncoa3; NCOA3_HUMAN; Nuclear receptor coactivator 3; pCIP; RAC 3; RAC-3; RAC3; Receptor associated coactivator 3; Receptor-associated coactivator 3; SRC 3; SRC-3; SRC3; Steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TNRC14; TNRC16; TRAM 1; TRAM-1; TRAM1. |
| Background | KAT13B/AIB1 interacts in a ligand-dependent fashion with the estrogen receptor to enhance estrogen-dependent transcriptional gene activation. The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits KAT3A / KAT3B as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-NCOA3 (Thr24) Polyclonal Antibody, FITC Conjugated (bs-3408R-FITC) validated for, and at what dilution?
bs-3408R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-NCOA3 (Thr24) Polyclonal Antibody, FITC Conjugated (bs-3408R-FITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Pig, Horse, Rabbit, GuineaPig.
How should bs-3408R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-NCOA3 (Thr24) Polyclonal Antibody, FITC Conjugated (bs-3408R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human SRC-3 around the phosphorylation site of Thr24
What are the excitation and emission wavelengths of the FITC conjugate (bs-3408R-FITC)?
FITC on bs-3408R-FITC: excitation / emission 494nm/518nm.