phospho-SHC1 (Tyr349) Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3411R-BF488-100uL |
| Product Name | phospho-SHC1 (Tyr349) Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human SHC around the phosphorylation site of Tyr349 |
| Modification Site | Tyr349 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6464 |
| Subcellular location | Cytoplasm |
| Synonyms | Shc1Tyr349; SHC1phospho Y349; p46; p52; p52 SHC; p52SHC; p66; p66 SHC; p66SHC; SH2 domain protein C1; SHC Src homology 2 domain containing transforming protein 1; SHC 1; SHC A; Shc; SHC transforming protein 1; SHC transforming protein; SHC1; SHCA; Src homology 2 domain containing transforming protein C1. |
| Background | The SHC gene encodes for a signaling and transforming protein containing Src homology 2 and 3 (SH2 and SH3) domains. The SHC gene encodes 2 widely expressed overlapping proteins of 46 and 52 kD, both containing a C-terminal SH2 domain. Adjacent to the SH2 region is a glycine and proline rich region. These 2 proteins differ in their N terminals. SHC proteins are involved in mitogenic signal transduction and act by coupling growth factor receptors to the RAS signaling pathway. The protein encoded by the SHC1 gene is thought to act as an adaptor in many signal transduction pathways. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is phospho-SHC1 (Tyr349) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3411R-BF488) validated for, and at what dilution?
bs-3411R-BF488 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-SHC1 (Tyr349) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3411R-BF488) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-3411R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-SHC1 (Tyr349) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3411R-BF488)?
KLH conjugated synthetic phosphopeptide derived from human SHC around the phosphorylation site of Tyr349
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-3411R-BF488)?
AbBy Fluor-488 on bs-3411R-BF488: excitation / emission 499nm/519nm.