Phospho-TORC2 (Ser171) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3415R-Cy7-100uL |
| Product Name | Phospho-TORC2 (Ser171) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Pig |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CREB regulated transcription coactivator 2 around the phosphorylation site of Ser171 |
| Modification Site | Ser171 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 200186 |
| Swiss Prot | Q53ET0 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | TORC2; TORC-2; CREB-regulated transcription coactivator 2; Transducer of regulated cAMP response element-binding protein 2; Transducer of CREB protein 2; CRTC2 |
| Background | Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-TORC2 (Ser171) Polyclonal Antibody, Cy7 Conjugated (bs-3415R-Cy7) validated for, and at what dilution?
bs-3415R-Cy7 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-TORC2 (Ser171) Polyclonal Antibody, Cy7 Conjugated (bs-3415R-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Pig.
How should bs-3415R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-TORC2 (Ser171) Polyclonal Antibody, Cy7 Conjugated (bs-3415R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human CREB regulated transcription coactivator 2 around the phosphorylation site of Ser171
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-3415R-Cy7)?
Cy7 on bs-3415R-Cy7: excitation / emission 743nm/767nm.