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LDHA Polyclonal Antibody

25 ug total protein per lane of various lysates (see on figure) probed with LDHA polyclonal antibody, unconjugated (bs-34202R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with LDHA polyclonal antibody, unconjugated (bs-34202R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (bs-34202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-F
  • IF
  • IHC-P

Reactivity

  • Human
Overview
Catalog # bs-34202R-100uL
Product Name LDHA Polyclonal Antibody
Applications
WB IHC-F IF IHC-P
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human LDHA
Clonality Polyclonal
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3939
Swiss Prot P00338
Subcellular location Cytoplasm
Synonyms Lactate Dehydrogenase; Lactate Dehydrogenase Isoenzyme V; Lactate Dehydrogenase isozyme H4; L lactate dehydrogenase A chain; Lactate dehydrogenase A; Lactate dehydrogenase A chain; LDH A; LDH heart subunit; LDH M; LDHM; LDH muscle subunit; LDH1; LDHA; PIG 19; PIG19; Proliferation inducing gene 19 protein; TRG 5; TRG5; LDHA_HUMAN.
Application Dilution
WB 1:500-2000
IHC-F 1:400-800
IF 1:10-50
IHC-P 1:100-500

Frequently asked questions

Which applications is LDHA Polyclonal Antibody (bs-34202R) validated for, and at what dilution?

bs-34202R is validated for WB (1:500-2000); IHC-F (1:400-800); IF (1:10-50); IHC-P (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does LDHA Polyclonal Antibody (bs-34202R) react with?

Tested reactivity: Human.

How should bs-34202R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise LDHA Polyclonal Antibody (bs-34202R)?

KLH conjugated synthetic peptide derived from human LDHA

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