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Phospho-TCTP (Ser46) Polyclonal Antibody, AbBy Fluor-647 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Horse
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-3422R-BF647-100uL
Product Name Phospho-TCTP (Ser46) Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Horse, Rabbit, GuineaPig
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human TCTP around the phosphorylation site of Ser46
Modification Site Ser46
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7178
Subcellular location Cytoplasm
Synonyms Fortilin; Histamine-releasing factor; p02; p23; TPT1; translationally controlled tumor protein; tumor protein translationally-controlled 1; TCTP_HUMAN.
Background TCTP is involved in calcium binding and microtubule stabilization. It is an immunoglobulin E dependent histamine-releasing factor produced by lymphocytes of atopic children and present in biologic fluids of allergic patients. Both human and mouse recombinant HRF proteins caused histamine release from human basophils of a subpopulation of donors (referenced from OMIM).
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Phospho-TCTP (Ser46) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3422R-BF647) validated for, and at what dilution?

bs-3422R-BF647 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-TCTP (Ser46) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3422R-BF647) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Horse, Rabbit, GuineaPig.

How should bs-3422R-BF647 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-TCTP (Ser46) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3422R-BF647)?

KLH conjugated synthetic phosphopeptide derived from human TCTP around the phosphorylation site of Ser46

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-3422R-BF647)?

AbBy Fluor-647 on bs-3422R-BF647: excitation / emission 650nm/665nm.

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