Phospho-TCTP (Ser46) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3422R-Cy5.5-100uL |
| Product Name | Phospho-TCTP (Ser46) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Horse, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human TCTP around the phosphorylation site of Ser46 |
| Modification Site | Ser46 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7178 |
| Subcellular location | Cytoplasm |
| Synonyms | Fortilin; Histamine-releasing factor; p02; p23; TPT1; translationally controlled tumor protein; tumor protein translationally-controlled 1; TCTP_HUMAN. |
| Background | TCTP is involved in calcium binding and microtubule stabilization. It is an immunoglobulin E dependent histamine-releasing factor produced by lymphocytes of atopic children and present in biologic fluids of allergic patients. Both human and mouse recombinant HRF proteins caused histamine release from human basophils of a subpopulation of donors (referenced from OMIM). |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-TCTP (Ser46) Polyclonal Antibody, Cy5.5 Conjugated (BS-3422R-CY5-5) validated for, and at what dilution?
BS-3422R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-TCTP (Ser46) Polyclonal Antibody, Cy5.5 Conjugated (BS-3422R-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Horse, Rabbit, GuineaPig.
How should BS-3422R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-TCTP (Ser46) Polyclonal Antibody, Cy5.5 Conjugated (BS-3422R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human TCTP around the phosphorylation site of Ser46
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-3422R-CY5-5)?
Cy5.5 on BS-3422R-CY5-5: excitation / emission 675nm/694nm.