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MAP2 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (bs-34252R) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (bs-34252R) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (bs-34252R) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (bs-34252R) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (bs-34252R) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (bs-34252R) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (bs-34252R) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (bs-34252R) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (bs-34252R) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (bs-34252R) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-34252r
Product Name MAP2 Polyclonal Antibody
Applications
IHC-P IHC-F IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MAP2
Clonality Polyclonal
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4133
Swiss Prot P11137
Subcellular location Cytoplasm, Nucleus
Synonyms Microtubule-associated protein 2; DKFZp686I2148; Dendrite specific MAP; DKFZp686I2148; MAP 2; MAP-2; MAP2A; MAP2B; MAP2C; Microtubule associated protein 2; Mtap 2; MAP2_HUMAN.
Background The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Application Dilution
IHC-P =1:300-1500
IHC-F IHC-F=1:300-1500
IF IF=1:300-1500

Frequently asked questions

Which applications is MAP2 Polyclonal Antibody (bs-34252R) validated for, and at what dilution?

bs-34252R is validated for IHC-P (IHC-P=1:300-1500); IHC-F (IHC-F=1:300-1500); IF (IF=1:300-1500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MAP2 Polyclonal Antibody (bs-34252R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-34252R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MAP2 Polyclonal Antibody (bs-34252R)?

KLH conjugated synthetic peptide derived from human MAP2

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