Alpha-actinin-1 Polyclonal Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Alpha-actinin-1 polyclonal antibody, unconjugated (bs-34256R) at 1:800 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. \r\n\r\n$

25 ug total protein per lane of various lysates (see on figure) probed with Alpha-actinin-1 polyclonal antibody, unconjugated (bs-34256R) at 1:800 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. \r\n\r\n

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-34256r
Product Name	Alpha-actinin-1 Polyclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated Synthetic peptide corresponding to Human alpha Actinin 1/ACTN1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	87
Swiss Prot	P12814
Subcellular location	Cytoplasm
Synonyms	ACTN 1; ACTN1_HUMAN; Alpha Actinin 1; Alpha actinin cytoskeletal isoform; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; FLJ40884; FLJ54432; Actinin Alpha 1; F-Actin Cross-Linking Protein; Non-Muscle Alpha-Actinin-1; Actinin, Alpha 1; Actinin 1 Smooth Muscle; BDPLT15;
Background	Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Application Dilution
WB	1:300-5000