Phospho-Stat2 (Tyr690) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3428R-Cy5.5-100uL |
| Product Name | Phospho-Stat2 (Tyr690) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to that of Tyr689 in Mouse and Tyr690 in Rat. |
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Horse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Stat2 around the phosphorylation site of Tyr690 |
| Modification Site | Tyr690 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6773 |
| Swiss Prot | P52630 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | P113; ISGF-3; STAT113; Signal transducer and activator of transcription 2; STAT2 |
| Background | Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Stat2 (Tyr690) Polyclonal Antibody, Cy5.5 Conjugated (BS-3428R-CY5-5) validated for, and at what dilution?
BS-3428R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Stat2 (Tyr690) Polyclonal Antibody, Cy5.5 Conjugated (BS-3428R-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Horse.
How should BS-3428R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Stat2 (Tyr690) Polyclonal Antibody, Cy5.5 Conjugated (BS-3428R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human Stat2 around the phosphorylation site of Tyr690
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-3428R-CY5-5)?
Cy5.5 on BS-3428R-CY5-5: excitation / emission 675nm/694nm.