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USP34 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human esophagus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (USP34) Polyclonal Antibody, Unconjugated (bs-34315R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human esophagus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (USP34) Polyclonal Antibody, Unconjugated (bs-34315R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (USP34) Polyclonal Antibody, Unconjugated (bs-34315R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (USP34) Polyclonal Antibody, Unconjugated (bs-34315R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • IHC-F
  • IF
  • IHC-P

Reactivity

  • Human

Predicted Reactivity

  • Mouse
Overview
Catalog # bs-34315r-100uL
Product Name USP34 Polyclonal Antibody
Applications
IHC-F IF IHC-P
Reactivity Human
Predicted Reactivity Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human USP34
Clonality Polyclonal
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 9736
Swiss Prot Q70CQ2
Subcellular location Cytoplasm, Nucleus
Synonyms Deubiquitinating enzyme 34 ; FLJ43910 ; KIAA0570 ; KIAA0729 ; MGC104459 ; Ubiquitin carboxyl terminal hydrolase 34 ; Ubiquitin carboxyl-terminal hydrolase 34 ; Ubiquitin specific processing protease 34 ; Ubiquitin specific protease 34 ; Ubiquitin thioesterase 34 ; Ubiquitin thiolesterase 34 ; Ubiquitin-specific-processing protease 34 ; UBP34_HUMAN ; USP 34 ; Usp34 ;
Application Dilution
IHC-F =1:100-500
IF IF=1:100-500
IHC-P IHC-P=1:100-500

Frequently asked questions

Which applications is USP34 Polyclonal Antibody (bs-34315R) validated for, and at what dilution?

bs-34315R is validated for IHC-F (IHC-F=1:100-500); IF (IF=1:100-500); IHC-P (IHC-P=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does USP34 Polyclonal Antibody (bs-34315R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.

How should bs-34315R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise USP34 Polyclonal Antibody (bs-34315R)?

KLH conjugated synthetic peptide derived from human USP34

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