Phospho-Syk (Tyr323) Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3433R-BF350-100uL |
| Product Name | Phospho-Syk (Tyr323) Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Syk around the phosphorylation site of Tyr323 |
| Modification Site | Tyr323 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6850 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | syk Tyr323; Tyrosine-protein kinase SYK Spleen tyrosine kinase [Contains: 72kDa tyrosine-protein kinase SYK; 40 kDa tyrosine-protein kinase SYK; KSYK_HUMAN. |
| Background | Syk (72 kDa) is a non receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions. Alternate Names: p72syk; Spleen tyrosine kinase; Tyrosine protein kinase SYK. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Phospho-Syk (Tyr323) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-3433R-BF350) validated for, and at what dilution?
bs-3433R-BF350 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Syk (Tyr323) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-3433R-BF350) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow.
How should bs-3433R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Syk (Tyr323) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-3433R-BF350)?
KLH conjugated synthetic phosphopeptide derived from human Syk around the phosphorylation site of Tyr323
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-3433R-BF350)?
AbBy Fluor-350 on bs-3433R-BF350: excitation / emission 346nm/442nm.