Phospho-Syk (Tyr525 + Tyr526) Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3434R-PerCP-100uL |
| Product Name | Phospho-Syk (Tyr525 + Tyr526) Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Syk around the phosphorylation site of Tyr525/526 |
| Modification Site | Tyr525 + Tyr526 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6850 |
| Swiss Prot | P43405 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | p72-Syk; Tyrosine-protein kinase SYK; Spleen tyrosine kinase; SYK |
| Background | This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Syk (Tyr525 + Tyr526) Polyclonal Antibody, PerCP Conjugated (bs-3434R-PerCP) validated for, and at what dilution?
bs-3434R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Syk (Tyr525 + Tyr526) Polyclonal Antibody, PerCP Conjugated (bs-3434R-PerCP) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Chicken, Rabbit, GuineaPig.
How should bs-3434R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Syk (Tyr525 + Tyr526) Polyclonal Antibody, PerCP Conjugated (bs-3434R-PerCP)?
KLH conjugated synthetic phosphopeptide derived from human Syk around the phosphorylation site of Tyr525/526
What are the excitation and emission wavelengths of the PerCP conjugate (bs-3434R-PerCP)?
PerCP on bs-3434R-PerCP: excitation / emission 482nm/677nm.