phospho-TOP2A (Ser1106) Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3451R-BF555-100uL |
| Product Name | phospho-TOP2A (Ser1106) Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Dog, Sheep, Pig, Zebrafish |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human TOPO II Alpha around the phosphorylation site of Ser1106 |
| Modification Site | Ser1106 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7153 |
| Subcellular location | Nucleus |
| Synonyms | DNA topoisomerasealpha; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme; TOP2; TP2A; TOP2A; DNA topoisomerase II 170 kD; DNA topoisomerase II alpha isozyme; DNA Topoisomerase2; TOP 2A; TopII alpha; Topo II alpha; Topoisomerase DNA II alpha 170kDa; Topoisomerase II alpha 170 kDa; TOP2A_HUMAN. |
| Background | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-TOP2A (Ser1106) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3451R-BF555) validated for, and at what dilution?
bs-3451R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-TOP2A (Ser1106) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3451R-BF555) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Sheep, Pig, Zebrafish.
How should bs-3451R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-TOP2A (Ser1106) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3451R-BF555)?
KLH conjugated synthetic phosphopeptide derived from human TOPO II Alpha around the phosphorylation site of Ser1106
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-3451R-BF555)?
AbBy Fluor-555 on bs-3451R-BF555: excitation / emission 553nm/568nm.