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TrkA (Tyr496) Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Phospho-TrkA (Tyr490)\/TrkB (Tyr516) Polyclonal Antibody, Unconjugated(bs-3456R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Phospho-TrkA (Tyr490)\/TrkB (Tyr516) Polyclonal Antibody, Unconjugated(bs-3456R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: mouse liver lysates Lane 2: mouse lung lysates probed with Anti Phospho-TrkA (Tyr490)\/TrkB (Tyr516) Polyclonal Antibody, Unconjugated (bs-3456R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 90kD. Observed band size: 90kD.
Lane 1: mouse liver lysates Lane 2: mouse lung lysates probed with Anti Phospho-TrkA (Tyr490)\/TrkB (Tyr516) Polyclonal Antibody, Unconjugated (bs-3456R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 90kD. Observed band size: 90kD.
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human K562 cell lysates probed with TrkA (Tyr496) Polyclonal Antibody, Unconjugated (bs-3456R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human K562 cell lysates probed with TrkA (Tyr496) Polyclonal Antibody, Unconjugated (bs-3456R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TrkA (Tyr496) Polyclonal Antibody(bs-3456R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TrkA (Tyr496) Polyclonal Antibody(bs-3456R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-3456R
Product Name TrkA (Tyr496) Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity This antibody will recognize TrkB when phosphorylated at Tyr516 and TrkC when phosphorylated at Tyr516
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human TrkA around the phosphorylation site of Tyr496
Modification Site Tyr496
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4914
Swiss Prot P04629
Subcellular location Cytoplasm, Cell membrane
Synonyms High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A; Trk-A; gp140trk; p140-TrkA; NTRK1; MTC; TRK; TRKA
Background Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors. Isoform TrkA-III is resistant to NGF, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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