Phospho-Cardiac Troponin I (Ser23 + Ser24) Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3458R-HRP |
| Product Name | Phospho-Cardiac Troponin I (Ser23 + Ser24) Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Specificity | These phosphorylation sites are homologous among the listed cross-reactive species. |
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Troponin I around the phosphorylation site of Ser23/24 |
| Modification Site | Ser23 + Ser24 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7138 |
| Swiss Prot | P13805 |
| Subcellular location | Cytoplasm |
| Synonyms | ANM; TNT; NEM5; STNT; TNTS; Troponin T, slow skeletal muscle; Slow skeletal muscle troponin T; TNNT1 |
| Background | Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Phospho-Cardiac Troponin I (Ser23 + Ser24) Polyclonal Antibody, HRP Conjugated (bs-3458R-HRP) validated for, and at what dilution?
bs-3458R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Cardiac Troponin I (Ser23 + Ser24) Polyclonal Antibody, HRP Conjugated (bs-3458R-HRP) react with?
Tested reactivity: Human, Rat.
How should bs-3458R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Cardiac Troponin I (Ser23 + Ser24) Polyclonal Antibody, HRP Conjugated (bs-3458R-HRP)?
KLH conjugated synthetic phosphopeptide derived from human Troponin I around the phosphorylation site of Ser23/24