Phospho-PLCG1 (Tyr771) Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3460R-BF594-100uL |
| Product Name | Phospho-PLCG1 (Tyr771) Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PLC gamma 1 around the phosphorylation site of Tyr771 |
| Modification Site | Tyr771 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5335 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | PLC 1; PLC 148; PLC gamma 1; PLC II; PLC1; PLC148; PLCG 1; PLCG1; PLCgamma1; PLCII; triphosphoinositide phosphodiesterase; 1 phosphatidyl D myo inositol 4 5 bisphosphate; 1 phosphatidylinositol 4 5 bisphosphate; 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; phosphatidylinositol phospholipase C; phosphodiesterase gamma 1; phosphoinositidase C; phosphoinositide phospholipase C; Phospholipase C 148; phospholipase C gamma 1 formerly subtype 148; Phospholipase C gamma 1; phospholipase C-148; PLCG1_HUMAN. |
| Background | The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-PLCG1 (Tyr771) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-3460R-BF594) validated for, and at what dilution?
bs-3460R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PLCG1 (Tyr771) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-3460R-BF594) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Horse.
How should bs-3460R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PLCG1 (Tyr771) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-3460R-BF594)?
KLH conjugated synthetic phosphopeptide derived from human PLC gamma 1 around the phosphorylation site of Tyr771
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-3460R-BF594)?
AbBy Fluor-594 on bs-3460R-BF594: excitation / emission 590nm/617nm.