Phospho-PPIG (Ser376) Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Phospho-PPIG (Ser376) Polyclonal Antibody, Unconjugated (bs-3461R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Testis lysates; Lane 2: Human HL-60 cell lysates probed with PPIG Polyclonal Antibody, Unconjugated (bs-3461R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Testis lysates; Lane 2: Human HL-60 cell lysates probed with PPIG Polyclonal Antibody, Unconjugated (bs-3461R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Pig
Horse
Chicken

Overview
Catalog #	bs-3461R
Product Name	Phospho-PPIG (Ser376) Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity	This phosphorylation site is homologous to that of Ser374 in Mouse and Rat.
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human PPIG around the phosphorylation site of Ser376 [VS(p-S)GE]
Modification Site	Ser376
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9360
Swiss Prot	Q13427
Subcellular location	Nucleus
Synonyms	CYP; SRCyp; SCAF10; CARS-Cyp; Peptidyl-prolyl cis-trans isomerase G; PPIase G; Peptidyl-prolyl isomerase G; CASP10; Clk-associating RS-cyclophilin; CARS-cyclophilin; SR-cyclophilin; SR-cyp; Cyclophilin G; Rotamase G; PPIG
Background	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200