Phospho-TH (Ser31) Polyclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3463R-APC |
| Product Name | Phospho-TH (Ser31) Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from rat TH around the phosphorylation site of Ser31 |
| Modification Site | Ser31 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7054 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Tyrosine Hydroxylase Ser31; Dystonia 14; DYT14; DYT5b; EC 1.14.16.2; OTTHUMP00000011225; OTTHUMP00000011226; ple; Protein Pale; TH; The; TY3H_RAT; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase. |
| Background | Neuronal MarkerTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-TH (Ser31) Polyclonal Antibody, APC Conjugated (bs-3463R-APC) validated for, and at what dilution?
bs-3463R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-TH (Ser31) Polyclonal Antibody, APC Conjugated (bs-3463R-APC) react with?
Tested reactivity: Mouse, Rat.
How should bs-3463R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-TH (Ser31) Polyclonal Antibody, APC Conjugated (bs-3463R-APC)?
KLH conjugated synthetic phosphopeptide derived from rat TH around the phosphorylation site of Ser31
What are the excitation and emission wavelengths of the APC conjugate (bs-3463R-APC)?
APC on bs-3463R-APC: excitation / emission 650nm/660nm.