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Phospho-TH (Ser31) Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse
  • Rat
Overview
Catalog # bs-3463R-Biotin
Product Name Phospho-TH (Ser31) Polyclonal Antibody, Biotin Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Mouse, Rat
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from rat TH around the phosphorylation site of Ser31
Modification Site Ser31
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 7054
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms Tyrosine Hydroxylase Ser31; Dystonia 14; DYT14; DYT5b; EC 1.14.16.2; OTTHUMP00000011225; OTTHUMP00000011226; ple; Protein Pale; TH; The; TY3H_RAT; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase.
Background Neuronal MarkerTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is Phospho-TH (Ser31) Polyclonal Antibody, Biotin Conjugated (bs-3463R-Biotin) validated for, and at what dilution?

bs-3463R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-TH (Ser31) Polyclonal Antibody, Biotin Conjugated (bs-3463R-Biotin) react with?

Tested reactivity: Mouse, Rat.

How should bs-3463R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-TH (Ser31) Polyclonal Antibody, Biotin Conjugated (bs-3463R-Biotin)?

KLH conjugated synthetic phosphopeptide derived from rat TH around the phosphorylation site of Ser31