Phospho-ATG1 (Ser556) Polyclonal Antibody, PE-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3464R-PE-Cy7-100uL |
| Product Name | Phospho-ATG1 (Ser556) Polyclonal Antibody, PE-Cy7 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat, Rabbit |
| Predicted Reactivity | Pig, Horse |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human ATG1 around the phosphorylation site of Ser556 |
| Modification Site | Ser556 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8408 |
| Swiss Prot | O75385 |
| Subcellular location | Cytoplasm |
| Synonyms | ATG1; ATG1A; UNC51; hATG1; Unc51.1; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; Unc-51-like kinase 1; ULK1; KIAA0722 |
| Background | Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-ATG1 (Ser556) Polyclonal Antibody, PE-Cy7 Conjugated (bs-3464R-PE-Cy7) validated for, and at what dilution?
bs-3464R-PE-Cy7 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-ATG1 (Ser556) Polyclonal Antibody, PE-Cy7 Conjugated (bs-3464R-PE-Cy7) react with?
Tested reactivity: Human, Mouse, Rat, Rabbit. Predicted reactivity (expected from sequence homology but not yet tested): Pig, Horse.
How should bs-3464R-PE-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-ATG1 (Ser556) Polyclonal Antibody, PE-Cy7 Conjugated (bs-3464R-PE-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human ATG1 around the phosphorylation site of Ser556
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bs-3464R-PE-Cy7)?
PE-Cy7 on bs-3464R-PE-Cy7: excitation / emission 488nm/780nm.